Identification of Meloidogyne javanica Using Morphological and Morphometerical Characters and Species Specific Primers

Askarian, H; SHarifnabi, B; Olia, M; Mahdikhani, E; Akhavan, A

Volume 13, Issue 47 (fall 2009) 2009, 13(47): 279-289 | Back to browse issues page

‎ 20.1001.1.22518517.1388.13.47.56.9

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Askarian H, SHarifnabi B, Olia M, Mahdikhani E, Akhavan A. Identification of Meloidogyne javanica Using Morphological and Morphometerical Characters and Species Specific Primers. Journal of Crop Production and Processing 2009; 13 (47) :279-289
URL: http://jcpp.iut.ac.ir/article-1-1055-en.html

Identification of Meloidogyne javanica Using Morphological and Morphometerical Characters and Species Specific Primers

H Askarian ^*

, B SHarifnabi

, M Olia

, E Mahdikhani

, A Akhavan

, homaaskarian@yahoo.com

Abstract: (27058 Views)

Root knot nematodes (Meloidogyne spp.) cause yield loss in all countries, of which, M. javanica, is the most widespread species in Iran. In order to identify M. javanica, 100 infected root and soil samples of root knot nematode were collected from different regions of Kerman province. After purification of populations and identification of M. javanica based on morphological and morphometerical characters of females and second stage juveniles (J2), total DNA was extracted from eggs, J2 and female adults. Specific 670 and 1600 bp bands were amplified in all M. javanica populations using species-specific primer pairs including OPARjav / OPAFjav and Mjavf / Mjavr These specific bands could not be amplified in other species such as M. incognita and M. arenaria. It seems that, application of these species specific primers in comparison with morphological characters would be more applicable, leading to easier identification of M. javanica.

Keywords: Root knot nematode, Meloidogyne javanica, Species specific primers, Morphology, Morphometry

Full-Text [PDF 514 kb] (1769 Downloads)

Type of Study: Research | Subject: General