Volume 5, Issue 2 (summer 2001)                   2001, 5(2): 27-37 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fotouhi Ghazvini R, Hammidoghli Y, Ebrahimi Y. Production of Virus-free Clones from Unfertilized Ovules of Satsuma Mandarin Cultivars. Journal of Crop Production and Processing 2001; 5 (2) :27-37
URL: http://jcpp.iut.ac.ir/article-1-159-en.html
Abstract:   (28831 Views)

In order to produce Tristeza virus-free plants from Satsuma mandarin (Citrus unshiu) cultivars (Ishikawa, Sugiyama and Myogawa), unfertilized ovules of plants suspected of CTV (Citrus Tristeza virus), from Mazandaran Mahdasht orchard were cultured on 4 media.

 Embryogenesis of nucellus tissue of ovules from Ishikawa and Sugiyama cultivars on MS medium were 31% and 25% and on MT medium from Ishikawa was 40.5%. Unfertilized ovules of Myogawa did not germinate on all media.

Nucellar embryos of Ishikawa and Sugiyama developed on MT+GA3+ME were 83% and 95%, respectively. Embryo differentiation, leaf and plantlet formation were observed on MT+GA3+ADE+ME medium. In the stage of two leaves, the plantlets’ growth under 1500 lux illumination was twice its growth under 800 lux illumination. Budding of in vitro plantlets from both cultivars on susceptible to Tristeza and virus-free seedlings of Key Lime did not show any infection by Tristeza.

Full-Text [PDF 4041 kb]   (1487 Downloads)    
Type of Study: Research | Subject: General

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.