RT - Journal Article T1 - Optimizing Regeneration of Cotton Plant via Shoot Apex JF - JCPP YR - 2004 JO - JCPP VO - 8 IS - 2 UR - http://jcpp.iut.ac.ir/article-1-431-en.html SP - 149 EP - 158 K1 - Cotton K1 - Shoot apex K1 - Regeneration. AB - Optimizing regeneration of cotton plant in two variety (Sahel and Varamin) via shoot apex was done in order to Agrobacterium-mediated transformation. In this reaserch shoot apexes of two varieties were isolated from four or five day seedling and were placed on a special medium of shooting (modified MS without hormon).In order to select the best rooting media, The Statistical Analysis explants that produce shoot and leaves in a CRBD with 4 replicates and 4 rooting treatments: 1) modified MS without hormon, 2) ½ MS with 0.1 mg/lit IBA, 3) ½ MS with 0.1 mg/lit NAA, 4) ½ MS with 0.1 mg/lit IAA. The statistical analysis indicated that the best for both varieties, was medium with 0.1 mg/lit IBA and rooting percentage of Varamin is better than sahel in all of media. LA eng UL http://jcpp.iut.ac.ir/article-1-431-en.html M3 ER -