Ethylene is an important contributor to the acceleration of fruit ripening and postharvest losses of fruit. Thus, anti ethylene substances could increase storage life of fruits. Thus the effects of 0 (control), 0.5, 0.75 and 1 µL L^-1 of 1-MCP and putrescine with 0 (control), 0.5, 1 and 1.5 mM on the storage life (1°C) during 21 day in strawberry fruit Cv. Selva were studied and appearance and the physicochemical characteristics of fruits were measured. A factorial experiment through complete randomized design (CRD) was used. The highest marketability, firmness, citric acid (TA), total soluble solids (TSS), vitamin C and the lowest amount of appearance decay, pH and the ratio of TSS/TA were observed in the treatment of 0.75 and 1 µL L^-1 of 1-MCP and 1 and 1.5 mM of putrescine. The results obtained from the experiment showed a positive effect of 1-MCP and putrescine on the storage life of strawberry. The results further showed that the use of 1-MCP and putrescine can act as an effective strategy in postharvest technology of strawberry fruit.