Volume 5, Issue 17 (12-2015)                   JCPP 2015, 5(17): 323-333 | Back to browse issues page

DOI: 10.18869/acadpub.jcpp.5.17.323

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zamiraee F, Bohlooli Zanjani S, Tarang A R, Kaviani B. Micropropagation of Lisianthus (Eustoma grandiflorum cv. White) at In Vitro Conditions. JCPP. 2015; 5 (17) :323-333
URL: http://jcpp.iut.ac.ir/article-1-2431-en.html

Islamic Azad University, Rasht, Iran. , b.kaviani@yahoo.com
Abstract:   (1036 Views)

Lisianthus (Eustoma grandiflorum cv. White) is an ornamental plant which has suitable marketing due to a variety of flower colours. The aim of the present study was to evaluate the effect of plant growth regulators on propagation and rooting of this plant. Axillary bud (node) was used as explants and MS medium supplemented with 0, 0.1, 0.2 and 2 mg/L of NAA and BA was applied. The results showed that the greatest shoot length (2.07 cm/plant) was obtained on a medium supplemented with 0.1 mg/L BA (without NAA). Maximum shoot number (5.80/plant) was produced in a medium containing 0.1 mg/L BA along with 0.2 mg/L NAA. Bud explants in culture media containing 0.2 mg/L NAA (without BA) and 0.1 mg/L NAA along with 2 mg/L BA produced the maximum node number (3.20/plant). The largest number of root (14.53/plant) and root length (3.87 cm/plant) were produced in media containing 0.2 mg/L NAA (without BA), also 0.2 mg/L BA plus 0.2 mg/L NAA and 0.2 mg/L BA without NAA, respectively. Explants produced flower on medium containing 0.1 mg/L BA along with 0.1 mg/L NAA at callus formation stage. Also, flower was produced from callus in medium containing 0.1 mg/L BA along with 2 mg/L NAA. Nearly 48% of the plantlets were able to survive on a medium containing a 1:1 ratio of peat and perlite.

Full-Text [PDF 483 kb]   (337 Downloads)    
Type of Study: Research | Subject: General

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author

© 2015 All Rights Reserved | Isfahan University of Technology - Journal of Crop Production and Processing

Designed & Developed by : Yektaweb

تحت نظارت وف بومی آسپا-وف