This research was conducted to investigate the changes of GA₃-like substances in the stratified (0, 10, 20, 30 days at 10^oC) and non-stratified seeds of two olive cultivars, namely ‘Arbequina’ and ‘Zard’. In the first experiment stratified seeds of these cultivars (10 g) were extracted with ethanol (80%), purified by ethyl acetate, and then separated by thin layer chromatography (TLC). To compare the biological activity of separated zones, lettuce hypocolyl assauy was used. The results obtained in this part of study indicated that the amount of GA₃-like substances increased as the stratification period increased. In the second experiment extracts were made from different parts of non-stratified seeds of both cultivars. The result of the second experiment showed that the GA₃–like substances of embryos of non-stratified seeds were higher than other parts of the seed.

Keywords: Stratification, GA₃-like substances, Chromatography, Olive seed.

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