Parsi Zira, Bunium persicum (Boiss.) B. Fedtsch., which is called Mountainous or Black Zira, is one of the most important medicinal plants with high economic importance. Generally, there is a little information about in vitro culture of Bunium persicum. Fragmented embryo was used as an explant in Bunium persicum regeneration. In this method, a great callus induction and regeneration only on the same medium without any subculture occurred because of being young and having better interaction with medium, leading to reduction of tissue culture time, infection and chemical consumption. In this research, B5 media containing different concentrations of plant growth regulators, NAA and 2,4-D only or together with Kin, were used. The experiment was conducted using completely randomized design with 30 treatments and 10 replications per treatment. The highest callus number was obtained from the treatment containing 0.1 mg/l 2,4-D and 2 mg/l Kin or 1 mg/l NAA and 2 mg/l Kin. Regeneration occurred in some treatments without Kinetin, showing that Kinetin is not essential for Bunium persicum regeneration. The treatment containing 0.1 mg/l NAA and 4 mg/l Kin was the best one for regeneration. The best treatment for somatic embryogenesis was 2 mg/l 2,4-D.