TY - JOUR T1 - Identification of AFLP Molecular Markers Linked to Row-Type Gene in Barley TT - شناسایی نشانگرهای AFLP پیوسته به ژن کنترل کننده تعداد ردیف بذر در جو JF - JCPP JO - JCPP VL - 8 IS - 4 UR - http://jcpp.iut.ac.ir/article-1-300-en.html Y1 - 2005 SP - 41 EP - 46 KW - Barley KW - Row-type KW - Near-isogenic lines (NILs) KW - aFLP KW - vrs1 N2 - Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrs1) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrs1 locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrs1 locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrs1 locus. M3 ER -