RT - Journal Article T1 - Polymorphism Syudy of Esterase, Proxidase and Malate Dehydrogenase Isozymes in Pistachio Cultivars and Related Species JF - JCPP YR - 2003 JO - JCPP VO - 7 IS - 1 UR - http://jcpp.iut.ac.ir/article-1-387-en.html SP - 107 EP - 114 K1 - Pistachio K1 - Isozyme K1 - Esterase K1 - Peroxidase K1 - Malate dehydrogenase K1 - Genetic diversity. AB - Thirty genotypes of pistachio cultivars and related species were evaluated for genetic diversity using three polymorphic isozymes, i.e. Esterase, Peroxidase and Malate dehydrogenase. Young leaves of pistachio were crushed with extraction buffer containing: 20% sucrose, 0.01 M dithiothretiol, 2% polyethylene glycol, and 8% polyvenyl polypyrollidone. Samples were analyzed using isoelectric focussing on polyacrylamide gels containing 2% (W/V) ampholyte. All the three isozymes revealed high degrees of polymorphism in pistachio cultivars and related species. Maximum polymorphism was observed for Est. enzyme. A total of 19 bands in Est. and 28 bands in MDH were observed in a wide range of pH gradient however, in Per. there were 11 bands all of which located in a narrow range of pH gradient. Cluster analysis based on the three system enzymes revealed that all the 30 pistachio genotypes were in 8 main classes and 20 subclasses and the extent of genetic similarity reduced from cultivated varieties to species, which were finally classified in 3 groups. Sarakhs variety, a wild type of P. vera, was classified in a group between cultivated varieties and species. LA eng UL http://jcpp.iut.ac.ir/article-1-387-en.html M3 ER -