TY - JOUR JF - JCPP JO - VL - 10 IS - 3 PY - 2006 Y1 - 2006/10/01 TI - Study of Genetic Diversity and Relationships of Diploid and Tetraploid Annual Medics Using Microsatellite Markers TT - بررسی تنوع ژنتیکی و روابط خویشاوندی یونجه‌های یک‌ساله دیپلوئید و تتراپلوئید با استفاده از نشانگرهای ریزماهواره N2 - Annual medics are used for hey production, soil protection, biological fixation of N2 and green manure. In the present study, the inter and intra specific genetic diversity and relatedness of 4 diploid and two tetraploid (M. rugosa and M. scutellata) annual medics were evaluated using microsatellite markers. PCR analysis was performed on genomic DNA from individual plant and PCR products were detected using standard polyacrylamide sequencing gel. Totally twenty five polymorphic alleles were observed in the studied species. Average intra-specific genetic diversity ranged from zero (0.0) in both M. rugosa and M. scutellata to 0.114 in M. minima species, and the level of genetic diversity was similar in both M. orbicularis and M. truncatula species. Analysis of molecular variance (AMOVA) was used to partition the overall genetic diversity into within and among species, and between diploids and tetraploids. The results revealed significant (P SP - 349 EP - 359 AU - M. Falahati-Anbaran, AU - A. A. Habashi, AU - M. Esfahany, AU - S. A. Mohammadi, AU - B. Ghareyazie, AD - KW - Annual medics ( Medicago spp.) KW - Genetic diversity KW - Microsatellite markers KW - Analysis of molecular variance (AMOVA). UR - http://jcpp.iut.ac.ir/article-1-589-en.html ER -