TY - JOUR T1 - Evaluation of Genetic Variation in Soybean Lines Using Seed Protein Electrophoresis TT - بررسی تنوع ژنتیکی لاین‌ های سویا با استفاده از روش الکتروفورز پروتئین دانه JF - JCPP JO - JCPP VL - 5 IS - 4 UR - http://jcpp.iut.ac.ir/article-1-85-en.html Y1 - 2002 SP - 85 EP - 96 KW - Cluster analysis KW - Correlation KW - Germplasm KW - Seed chemical composition. N2 - This experiment was conducted to study genetic variation for electrophoretic seed protein patterns and their relations with some seed characteristics such as protein and oil percentages, chemical compositions and 100-seed weight among 270 soybean (Glycine max L. Moench) genotypes. Among different electrophoresis procedures examined, 10% and 4.5% concentrations of acrylamide for resolving and stacking gels, respectively, 13.5 mg/ml concentration of protein buffer extraction, injection of 10 microlitre sample injection into gel hollows, 2.5 miliamper and 2-hours staining period were determined as the best combination to achieve clear bands and good separation. Based on relative mobility on gel, 30 protein bands were observed, of which only 5 varied among genotypes. In general, 8 electrophoretic patterns were recognized. Cluster analysis based on qualitative evaluation of patterns grouped the genotypes in 8 clusters and classified different bands in three groups. Simple concordance (matching) coefficients between protein bands with relative mobilities of 2.5% and 49.5% were zero, which is an indication of their independent occurrence. Probably, these bands are coded with one gene that in dominant and recessive homozygous genotypes appear as a single band at different positions on the gel. Analysis of variance revealed significant differences (P M3 ER -