F. Ahmadloo, M. Tabari Kouchaksaraei, P. Azadi, A. Hamidi, E. Beiramizadeh,
Volume 5, Issue 17 (12-2015)
Abstract
Crataegus is a tree species from Rosaceae family with medicinal, ornamental and commercial utilizations. Effect of different concentrations of plant growth regulators on shoot proliferation of Crataegus pseudohetrophylla Pojark. via in vitro culture was studied using single node explants. The grown shoots were transferred to MS medium supplemented with different concentrations of cytokinins (BAP, KIN, 2iP and Zt) in combination with NAA in 30 treatments, with 3 replications and each replication included 5 explants. Data were recorded after 3 subcultures. For rooting, basal end of shoots were dipped into 300mg/l of IBA at different durations (0, 5, 10, 20, 30, 40, 50 and 60 minutes), then the dipped shoots were cultured on hormone free 1/2 VS medium. The results of analysis of variance showed that there were significant differences in all of the traits. Results indicated that the highest number of shoots (39.33) was obtained on medium containing 8 mg / l BAP plus 2 mg / l NAA. The highest shoot length (4.67 cm) and leaf size (3.73 cm) was achieved on 7 mg / l BAP plus 2 mg / l NAA and 2 mg / l Zt plus 1 mg / l NAA, respectively. The highest rate of rooting (33.33%) and root number (4n) were induced on shoots dipped for 40 minutes in 300 mg / l IBA.
Z. Khazaei Kojori, M. Rezaei, Sh. Gharangik, H. Ghorbani,
Volume 6, Issue 19 (5-2016)
Abstract
In vitro propagation of three Iranian apricot cultivars, “Ghavami”, “RajabAli” and “Khiveei” was studied by direct micropropagation technique. To optimize sterile manipulation, shoot nods of one year-old dormant vegetative shoots and current growing vegetative shoot were cultured in WPM medium after treatment by HgCl2, citric acid, ethanol and NaClO (10, 15, 20 min). Establishment of buds was tested in the MS and WPM mediums. Proliferation of sprouting buds was evaluated in WPM medium supplemented with three concentrations of (0.5, 1 and 2 mg/l) BAP and 0.05 mg/l of IBA. A half-Strength MS medium supplemented with three concentrations (0.5, 1 and 2 mg/l) of IBA was used as a rooting medium. The greatest number of contamination-free shoots in HgCl2 0.01% and citric acid 0.07% treatments were obtained. No significant difference was found between two different mediums in terms of bud vegetative growth. The shoot number and length were significantly affected by apricot varieties and BAP concentrations. The greatest numbers of proliferated shoot were observed in Rajabali and Ghavami varieties at 1 mg/l of BAP, whereas maximum number of shoot in Khiveei cultivar was observed in 0.5 mg/l of BAP. The maximum shoot length in Rajabali and Ghavami cultivars was obtained in 2 mg/l of BAP.
M. Kabiri, S. A. M. Mirmohammady Maibody, A. Shakib, A. Rezaei,
Volume 9, Issue 4 (1-2006)
Abstract
To obtain a suitable explant and efficient culture medium for plant regeneration in spinach, two cultivars of Melody and Karaj local seedlings were chosen. A hypocotyl and cotyledon segments as well as shoot tip explants were dissected from seedlings. The explants were then cultured on MS medium supplemented with IAA, GA3, NAA, and BAP and their response to this media was studied. A completely randomized design with different replicates was used to conduct the experiments. Callus was formed at the base of the hypocotyl explants on the medium containing 15 mg.l-1 IAA, and 3.4 mg.l-1 GA3. Calli capable of regeneration was obtained after subculturing on the medium containing 2 mg.l-1 IAA, and 3.4 mg.l-1 GA3 at the rate of 38 percent. The vitrified plantlets were abnormally glassy, and translucent which might have high water content. It was a physiological disorder which was overcome when an improved agar medium raising to 9 gl-1 was used. Callus has been obtained only from the hypocotyl explants, while regeneration has been obtained from shoot tip cultured on the medium containing 0.02 mgl-1 BAP at the rate of 80 percent.
