S. Zafarian, S. Houshmand,
Volume 3, Issue 8 (6-2013)
Abstract
Karafs Kouhi (Kelussia odoratissima M.) is one of the important medicinal plants of umbelliferae family and native of Zagros Mountains range, which is endangered due to illegal harvests. In order to accelerate breaking the seed dormancy of this plant, a factorial experiment based on completely randomized design with four replications, was carried in Faculty of Agriculture, Shahrekord University. The seeds of Karafs Kouhi from Saraghaseyed ecotype were treated under three time periods (25, 50, 75 and 100 days after planting), combination of enzylaminopurine (BAP) (concentrations of 0, 0.75 and 1.5 mg/L) and gibberellic acid (GA3) (concentrations of 0, 250 and 500 mg/L) and application methods of these growth regulators (soaking seeds in the growth regulators and use of growth regulators directly on the medium). In this experiment, germination percentage, rootlet length and hypocotyl length were studied. The results indicated that 100 days after planting significantly (P≥0.01) showed the highest rates in the three studied traits. In this treatment, the germination percentage was 86.94%, rootlet length was 9.43 cm and hypocotyl length was 11.64 cm. Moreover, the interaction of the factors was not significant for all the traits. The best combination to increase germination percent, rootlet length and germination rate, was 0.75 mg/L BAP, 500 mg/L GA3 and direct use of growth regulators. To increase hypocotyl length, only 500 mg/L GA3 is recommended.
M. Kabiri, S. A. M. Mirmohammady Maibody, A. Shakib, A. Rezaei,
Volume 9, Issue 4 (1-2006)
Abstract
To obtain a suitable explant and efficient culture medium for plant regeneration in spinach, two cultivars of Melody and Karaj local seedlings were chosen. A hypocotyl and cotyledon segments as well as shoot tip explants were dissected from seedlings. The explants were then cultured on MS medium supplemented with IAA, GA3, NAA, and BAP and their response to this media was studied. A completely randomized design with different replicates was used to conduct the experiments. Callus was formed at the base of the hypocotyl explants on the medium containing 15 mg.l-1 IAA, and 3.4 mg.l-1 GA3. Calli capable of regeneration was obtained after subculturing on the medium containing 2 mg.l-1 IAA, and 3.4 mg.l-1 GA3 at the rate of 38 percent. The vitrified plantlets were abnormally glassy, and translucent which might have high water content. It was a physiological disorder which was overcome when an improved agar medium raising to 9 gl-1 was used. Callus has been obtained only from the hypocotyl explants, while regeneration has been obtained from shoot tip cultured on the medium containing 0.02 mgl-1 BAP at the rate of 80 percent.
