M. Solgi, M. Taghizadeh,
Volume 5, Issue 17 (12-2015)
Abstract
One of the major advantages of in vitro culture of African violet (Saintpaulha ionantha) is production of new cultivars and propagation of their chimera which might not be propagated by the other methods. In this study, we tested the effects of silver nanoparticles on the sterilization rate (antifungal and antibacterial activity), regeneration and shoot formation of African violet "Pink Amiss" explants. These nanoparticles were synthesized from pomegranate peels and Damask rose petals extracts. We used a completely randomized design test with factorial arrangement to investigate various volumetric ratios of plant extracts to silver nitrate (1:20, 1:10, 1:5 and 1:1) on the culture contaminations. Using silver nanoparticles synthesized by the plant extracts, especially Damask rose petals extract resulted in no fungal and bacterial contamination in the African violet explants after 1 and 3 weeks as compared to the control, and silver nitrate (1mM). All tested concentrations of the silver nanoparticles significantly (P &le 0.05) controlled both bacterial and fungal contaminations. The 1:20 ratio of plant extracts to silver nitrate showed the best control. In addition, the highest regeneration (%52) and shoot regeneration (%38) was observed in this treatment. In conclusion, we suggest using silver nanoparticles synthesized by plant extracts for sterilization of in Vitro Culture for African Violet rather than using other chemicals such as silver nitrate.
A. Sarkhosh, Z. Zamani, M. R. Fatahi Moghaddam, A. Ebadi, A. Saie, S. Z. Tabatabaie, M. R. Akrami,
Volume 10, Issue 4 (1-2007)
Abstract
In order to determine the most important quantitative and qualitative characteristics of pomegranate fruit and its components for categorizing the genotypes, a study was carried out using 24 genotypes. In this study 28 quantitative and qualitative characteristics of pomegranate fruit and seed were evaluated. Analysis of variance showed that all of the characteristics in examined genotypes were significant, showing high variability in characteristics. Results of simple correlation analysis showed the existence of significant, positive and negative correlations among some important characteristics. Also, factor analysis showed that most of fruit juice, aril and seed characteristics constituted the main factors. Effective characteristics categorized in seven factors accounted for 89% of total variance. For each factor, eigen value more than 0.7 was considered significant. Cluster analysis was performed using these seven factors and genotypes, in the distance of 9, were divided into 5 main clusters. These groups were mainly distinct in fruit flavor and soft seededness. Furthermore, by using three main factors, genotypes positions determined in 3-plot analysis, which distinguished sweeter genotypes from sweet-sour and sour genotypes.
