Volume 5, Issue 17 (12-2015)                   2015, 5(17): 349-358 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Solgi M, Taghizadeh M. Sterilization of African Violet in the in Vitro Culture Using Synthesized Silver Nanoparticles by Two Plant Extracts. Journal of Crop Production and Processing 2015; 5 (17) :349-358
URL: http://jcpp.iut.ac.ir/article-1-2434-en.html
Arak University,Arak, Iran. , ir.ac.araku@solgi-M
Abstract:   (3498 Views)

One of the major advantages of in vitro culture of African violet (Saintpaulha ionantha) is production of new cultivars and propagation of their chimera which might not be propagated by the other methods. In this study, we tested the effects of silver nanoparticles on the sterilization rate (antifungal and antibacterial activity), regeneration and shoot formation of African violet "Pink Amiss" explants. These nanoparticles were synthesized from pomegranate peels and Damask rose petals extracts. We used a completely randomized design test with factorial arrangement to investigate various volumetric ratios of plant extracts to silver nitrate (1:20, 1:10, 1:5 and 1:1) on the culture contaminations. Using silver nanoparticles synthesized by the plant extracts, especially Damask rose petals extract resulted in no fungal and bacterial contamination in the African violet explants after 1 and 3 weeks as compared to the control, and silver nitrate (1mM). All tested concentrations of the silver nanoparticles significantly (P &le 0.05) controlled both bacterial and fungal contaminations. The 1:20 ratio of plant extracts to silver nitrate showed the best control. In addition, the highest regeneration (%52) and shoot regeneration (%38) was observed in this treatment. In conclusion, we suggest using silver nanoparticles synthesized by plant extracts for sterilization of in Vitro Culture for African Violet rather than using other chemicals such as silver nitrate.

Full-Text [PDF 265 kb]   (1173 Downloads)    
Type of Study: Research | Subject: General

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.