Volume 11, Issue 41 (fall 2007)                   2007, 11(41): 373-380 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

S. Esmail Khanian, A. Negati Javaremi, F. Afraz, P. Daneshyar, S. Ghanbari. Genetic Variation among Baluchi Sheep Population Using Microsatellite Markers . Journal of Crop Production and Processing 2007; 11 (41) :373-380
URL: http://jcpp.iut.ac.ir/article-1-752-en.html
Abstract:   (24803 Views)
In order to identify polymorphic microsatellite markers and evaluae genetic variation within Baluchi sheep population, nineteen microsatellite loci were studied. Whole Blood samples were collected from 156 sheep at north eastern animal breeding station of Iran (Abbasabad-Mashhad). DNA was extracted by salting-out procedure with some modifications. Polymerase chain reactions were successfully done except for UNC5C locus. PCR products were electrophoresed on 8% denaturing polyacrylamide gels stained according to rapid silver staining procedure. The genotype and allelic frequencies were calculated by direct counting and used for estimating of different polymorphism and genetic variation criteria. This population wasn't at Hardy-Weinberg equilibrium except for OarAE101 locus (P<0.005). Heterozygosity (gene variation) ranged from 0.1 to 0.93. BULGE5E and BM1329 loci were monomorphic. In conclusion, this investigation showed high polymorphism at the studied loci, so they could be used in future studies.
Full-Text [PDF 471 kb]   (1840 Downloads)    
Type of Study: Research | Subject: General

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.