In order to define a suitable sowing date and effect of nitrogen fertilizer on quinoa, a randomized complete block design field experiment was conducted based on a split-plot design with three replications in 2019 at Ahar, Iran. Experimental treatments included: Five sowing dates (Feb. 24, Mar. 11, Mar. 25, Apr. 9 and Apr. 25) as main plot and five levels of nitrogen fertilizer (0, 90, 180, 270 and 360 kg ha-1) as sub plots. Results indicated that maximum 1000-seed weight (3.48 g) was obtained at sowing date of 25 Apr. under 180 kg ha-1 nitrogen and the highest grain yield and biomass were observed at sowing date of 25 Apr. with application of 270 and 360 kg ha-1 nitrogen fertilizer. The highest plant height and grain nitrogen percentage were obtained in the sowing date of 25 Apr. with application of 90, 180, 270 and 360 kg ha-1. A significant increase in nitrogen agronomic efficiency was observed with increasing application of nitrogen from control to 90 kg ha-1 in all sowing dates. The highest growing period (with 137 days) was observed at sowing date of 24 Feb. and the lowest growing period (with 95 days) was observed at sowing date of 25 Apr. Generally, the sowing date of 25 Apr. with application of 270 kg ha-1 nitrogen fertilizer is suitable for Ahar region owing to the shortest growing period and highest grain yield and biomass.